Label Free Biosensors

Author: Matthew A. Cooper
Editor: Cambridge University Press
ISBN: 0521884535
File Size: 15,61 MB
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A detailed technical review of label-free biosensor techniques with worked examples.

Label Free Biosensing

Author: Michael J. Schöning
Editor: Springer
ISBN: 3319752200
File Size: 68,83 MB
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This volume summarizes the state-of-the-art technologies, key advances and future trends in the field of label-free biosensing. It provides detailed insights into the different types of solid-state, label-free biosensors, their underlying transducer principles, advanced materials utilized, device-fabrication techniques and various applications. The book offers graduate students, academic researchers, and industry professionals a comprehensive source of information on all facets of label-free biosensing and the future trends in this flourishing field. Highlights of the subjects covered include label-free biosensing with: · semiconductor field-effect devices such as nanomaterial-modified capacitive electrolyte-insulator-semiconductor structures, silicon nanowire transistors, III-nitride semiconductor devices and light-addressable potentiometric sensors · impedimetric biosensors using planar and 3D electrodes · nanocavity and solid-state nanopore devices · carbon nanotube and graphene/graphene oxide biosensors · electrochemical biosensors using molecularly imprinted polymers · biomimetic sensors based on acoustic signal transduction · enzyme logic systems and digital biosensors based on the biocomputing concept · heat-transfer as a novel transducer principle · ultrasensitive surface plasmon resonance biosensors · magnetic biosensors and magnetic imaging devices

Label Free Biosensor Methods In Drug Discovery

Author: Ye Fang
Editor: Humana Press
ISBN: 9781493949618
File Size: 10,75 MB
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This volume explores label-free biosensors, advantageous in part because this technology bypasses the need of labels, reporters, and cell engineering, all of which are common to labeled techniques but may introduce artifacts in assay results. Addressing several fundamental and practical aspects as to how to implement label-free methods in the drug discovery process, this book covers a wide range of topics, including binding kinetics determination, fragment screening, antibody epitope mapping, protein-protein interaction profiling and screening, receptor pathway deconvolution, drug pharmacology profiling and screening, target identification, drug toxicity assessment, and physical phenotype profiling and diagnostics based on various cellular processes such as cell adhesion, migration, invasion, infection, and inflammation. As part of the Methods in Pharmacology and Toxicology series, chapters aim to provide key detail and implementation advice to aid with progress in the lab. Practical and thorough, Label-Free Biosensor Methods in Drug Discovery provides a new avenue for rapid access to a focused collection of highly regarded contributions in the field.

Study And Development Of Label Free Optical Biosensors For Biomedical Applications

Author: Charles J. Choi
Editor:
ISBN:
File Size: 70,95 MB
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For the majority of assays currently performed, fluorescent or colorimetric chemical labels are commonly attached to the molecules under study so that they may be readily visualized. The methods of using labels to track biomolecular binding events are very sensitive and effective, and are employed as standardized assay protocol across research labs worldwide. However, using labels induces experimental uncertainties due to the effect of the label on molecular conformation, active binding sites, or inability to find an appropriate label that functions equivalently for all molecules in an experiment. Therefore, the ability to perform highly sensitive biochemical detection without the use of fluorescent labels would further simplify assay protocols and would provide quantitative kinetic data, while removing experimental artifacts from fluorescent quenching, shelf-life, and background fluorescence phenomena. In view of the advantages mentioned above, the study and development of optical label-free sensor technologies have been undertaken here. In general, label-free photonic crystal (PC) biosensors and metal nanodome array surface-enhanced Raman scattering (SERS) substrates, both of which are fabricated by nanoreplica molding process, have been used as the method to attack the problem. Chapter 1 shows the work on PC label-free biosensor incorporated microfluidic network for bioassay performance enhancement and kinetic reaction rate constant determination. Chapter 2 describes the work on theoretical and experimental comparison of label-free biosensing in microplate, microfluidic, and spot-based affinity capture assays. Chapter 3 shows the work on integration of PC biosensor with actuate-to-open valve microfluidic chip for pL-volume combinatorial mixing and screening application. In Chapter 4, the development and characterization of SERS nanodome array is shown. Lastly, Chapter 5 describes SERS nanodome sensor incorporated tubing for point-of-care monitoring of intravenous drugs and metabolites.

Label Free Sensing Of Chemical And Biological Interactions Using Nanomechanics And Nanobiomimetics

Author: Digvijay Ashokkumar Raorane
Editor:
ISBN:
File Size: 16,45 MB
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Electrochemical Dna Biosensors

Author: Mehmet Sengun Ozsoz
Editor: CRC Press
ISBN: 9814241776
File Size: 80,75 MB
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This book focuses on the basic electrochemical applications of DNA in various areas, from basic principles to the most recent discoveries. The book comprises theoretical and experimental analysis of various properties of nucleic acids, research methods, and some promising applications. The topics discussed in the book include electrochemical detection of DNA hybridization based on latex/gold nanoparticle and nanotubes; nanomaterial-based electrochemical DNA detection; electrochemical detection of microorganism-based DNA biosensors; gold nanoparticle-based electrochemical DNA biosensors; electrochemical detection of the aptamer-target interaction; nanoparticle-induced catalysis for DNA biosensing; basic terms regarding electrochemical DNA (nucleic acids) biosensors; screen-printed electrodes for electrochemical DNA detection; application of field-effect transistors to label free electrical DNA biosensor arrays; and electrochemical detection of nucleic acids using branched DNA amplifiers.

Performance Improvement Of Label Free Biosensors And Their Applications In Dna And Protein Microarrays

Author: Sunmin Ahn
Editor:
ISBN:
File Size: 18,74 MB
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Abstract: Microarray technologies have provided powerful tools in modern biotechnology to decipher the complex interconnectivity of genetic and regulatory networks. Label-free biosensors have emerged as competitive technologies because they offer more economic and simple procedures, allow molecular interactions in their native state, and provide interaction kinetics, compared to the traditional luminescence-based sensors. While these advantages enable better understandings of the intricate biomolecular interactions, label-free biosensors have yet to be widely adapted in biological and medical research.We present two approaches to develop successful, high-throughput, label-free biosensors by using Interferometric Reflectance Imaging Sensor (IRIS). First, we offer strategies to improve the performance of label-free biosensors by increasing sensitivity and accuracy. Sensitivity enhancement is achieved by utilizing mass tags. Accuracy improvement is accomplished through extensive characterization of stability of DNA and protein microarrays. Following stability characterization of the surface chemistry, the substrate, and the immobilized biomolecules, microarray fabrication methods and normalization techniques are developed to reduce error, hence, increasing the accuracy of quantitative analysis. The degradations of the sensor surface that we discover from stability characterization are susceptible to other label-free biosensors. Thus, the correction strategies that we present can be utilized for accurate quantitative analysis of a variety of label-free biosensors. Second, we develop four applications for IRIS. 1) We present applications for quantitative gene expression analysis and disease screening using DNA microarrays by demonstrating quantitative analysis of DNA hybridization and successful detection of single nucleotide polymorphism. 2) We present an application for quantitative analysis of transcription factors using ssDNA and dsDNA microarrays. We discover a new binding motif for TATA-binding protein and propose alternative models for eukaryotic transcription initiation. 3) We present an application to study immune response using antibody microarrays by demonstrating dynamic detection interleukin-6 with ∼ ng/mL sensitivity and successfully detecting the small macromolecule in biologically complex fluid. 4) We propose an application to investigate cellular response to external stimuli, such as drugs, toxins, and pathogens, using patterned cell-protein microarrays. We present strategies of fabricating cell-protein microarrays and demonstrate interleukin-8 detection with ∼ pg/mL sensitivity using gold nanoparticles. Improved performance and diverse biological applications of IRIS will help successful implementations of high-throughput label-free biosensors in healthcare.

Biosensors For Health Environment And Biosecurity

Author: Pier Andrea Serra
Editor: IntechOpen
ISBN: 9789533074436
File Size: 44,86 MB
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A biosensor is a detecting device that combines a transducer with a biologically sensitive and selective component. Biosensors can measure compounds present in the environment, chemical processes, food and human body at low cost if compared with traditional analytical techniques. This book covers a wide range of aspects and issues related to biosensor technology, bringing together researchers from 16 different countries. The book consists of 24 chapters written by 76 authors and divided in three sections: Biosensors Technology and Materials, Biosensors for Health and Biosensors for Environment and Biosecurity.

Handbook Of Graphene

Author: Barbara Palys
Editor: John Wiley & Sons
ISBN: 1119469767
File Size: 38,24 MB
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The sixth volume in a series of handbooks on graphene research and applications The Handbook of Graphene, Volume 6: Biosensors and Advanced Sensors discusses the unique benefits that the discovery of graphene has brought to the sensing and biosensing sectors. It examines graphene's use in leading-edge technology applications and the development of a variety of graphene-based sensors. The handbook looks at how graphene can be used as an electrode, substrate, or transducer in sensor design. Graphene-based sensor detection has achieved up to femto-levels, with performances delivering the advantages of greater selectivity, sensitivity, and stability.

Numerical Characterisation Of Label Free Optical Biosensors

Author: T. Dar
Editor:
ISBN:
File Size: 18,47 MB
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External Cavity Laser Biosensor For Label Free Detection

Author:
Editor:
ISBN:
File Size: 64,72 MB
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Development Of Lspr Based Optical Biosensors For The Label Free Detection Of Biomolecular Interactions In High Density Peptide Arrays

Author: Fanny Caroline Liu
Editor:
ISBN:
File Size: 27,81 MB
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Optics Letters

Author:
Editor:
ISBN:
File Size: 45,96 MB
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Label Free Detection Of Tuberculosis Dna With Capacitive Field Effect Biosensors

Author: Thomas Stefan Bronder
Editor:
ISBN:
File Size: 58,22 MB
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Handbook Of Graphene

Author: Barbara Palys
Editor: John Wiley & Sons
ISBN: 1119469759
File Size: 79,53 MB
Format: PDF, Kindle
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The sixth volume in a series of handbooks on graphene research and applications The Handbook of Graphene, Volume 6: Biosensors and Advanced Sensors discusses the unique benefits that the discovery of graphene has brought to the sensing and biosensing sectors. It examines graphene's use in leading-edge technology applications and the development of a variety of graphene-based sensors. The handbook looks at how graphene can be used as an electrode, substrate, or transducer in sensor design. Graphene-based sensor detection has achieved up to femto-levels, with performances delivering the advantages of greater selectivity, sensitivity, and stability.

Label Free Plasmonic Biosensors For Real Time Live Cell Analysis

Author: Xiaokang Li
Editor:
ISBN:
File Size: 75,72 MB
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Mots-clés de l'auteur: label-free biosensor ; nanoplasmonics ; nanohole arrays ; surface plasmon resonance ; live cell analysis ; single-cell analysis ; cell secretion ; lipid membrane ; TCR affinity analysis.

Australian Journal Of Chemistry

Author:
Editor:
ISBN:
File Size: 50,40 MB
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Advancing The Biosensing Technologies Of Two Optical Label Free Sensing Platforms

Author: Rashmi Sriram
Editor:
ISBN:
File Size: 46,64 MB
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"Optical label-free biosensors promise high-throughput, rapid, and sensitive detection of biomolecules. As such, these biodetection platforms have applications in clinical diagnostics, pharmaceutical industry, homeland security and in environmental monitoring. Motivated by these attributes, my thesis has focused on advancing the biosensing technology of two novel silicon-based optical label-free sensors: arrayed imaging reflectometry (AIR) and two-dimensional photonic crystal structures (2D PhC). The AIR biosensor is an interferometry-based technique that relies on the creation and destruction of a near-zero reflectivity condition in a carefully chosen thin film. To thoroughly quantify the biosensor detection signal, a correlation between the sensor response and the solution analyte concentration needs to be determined. In the first half of this thesis, a theoretical framework was developed to predict the AIR sensor response for a given solution analyte concentration. The developed model was subsequently validated using spectroscopic ellipsometry and surface plasmon resonance techniques in addition to AIR by utilizing two model probe-analyte pairs. It was established that the probe-analyte interaction on AIR substrate across two formats: macrospots and microarrays closely represented one-site and two-site Langmuir models, respectively. Two-dimensional photonic crystals are periodic dielectric structures with spatial periodicity in two dimensions. In the presence of a "defect" microcavity that disrupts the spatial periodicity, light of specific wavelength can be captured within the defect cavity. This manifests as a "dip" in the transmission spectrum of the crystal and is very sensitive to the local refractive index changes in the immediate vicinity of the defect cavity. The second half of this thesis investigates the utility of two designs of the 2D PhC: "small" and "large" point-defect microcavities coupled to a transmission waveguide for sensing latex particles in microfluidic environment. With the "small" defect design, detection of ~100 nm size latex particles in microfluidic environment was confirmed. This is the first study that demonstrates particle detection in the presence of a fluid cover using a 2D PhC sensor, a significant technological advancement for the incorporation of this sensor into lab-on-a-chip device. Using a "large" point-defect cavity design, real-time detection of latex particles of size ~280 nm was demonstrated. This to our knowledge is the first demonstration of detection of latex particles in real-time using 2D PhCs without the aid of any optical forces. This proof-of-principle demonstration of particle detection lays the foundation for the further development of these devices for the detection of a single biological particle, which when accomplished will have a huge impact in the field of clinical diagnostics"--Pages ix-x.

Label Free Biosensors

Author: Martin Nirschl
Editor: Sudwestdeutscher Verlag Fur Hochschulschriften AG
ISBN: 9783838131658
File Size: 29,58 MB
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Thin-film bulk acoustic resonators are microfabricated devices which can detect mass changes on their surface with high sensivity. With a specific functionalisation immobilised on the suface, the FBARs can be used as biosensors. Resulting from their small size they show highly interesting properties such as low liquid consumption, the large number of resonators that can be used in parallel, the high measurement speed and the possibility of detecting conformational changes of biomolecules such as proteins. With these features, it seems to be likely that there are applications for which the FBAR is superior to the other established technologies. One of these applications is the analysis of biomolecular interactions. This book gives a summary of the measurement technogies commercially used for label-free biomolecular interaction analysis and presents the advantages and disadvantages of the FBAR in theory and application.